Sysmex XF-1600 Analytical Platform: Technological Excellence in Multicolour Flow Cytometry
Sysmex XF-1600 is a high-tech solution for multiparametric cell analysis, combining advanced optics, stable fluidics, and intelligent software. Engineered for high-throughput environments, the system ensures exceptional reproducibility for even the most complex diagnostic panels.
Professional List of Applications
The system enables a wide range of protocols through its 10-colour (expandable) panel support:
- Hemoblastosis Immunophenotyping: Comprehensive diagnostics of acute and chronic leukemias and lymphomas.
- Immune Status Assessment: Advanced analysis of T, B, and NK cell subpopulations (including naive and memory cells).
- Minimal Residual Disease (MRD) Detection: High-sensitivity detection of aberrant phenotypes post-therapy.
- PNH Diagnostics: High-sensitivity assays for Paroxysmal Nocturnal Hemoglobinuria according to ICCS/ESCP protocols.
- DNA and Cell Cycle Analysis: Quantitative determination of DNA content and cell division phases.
- Functional Assays: Phagocytic activity, cytokine determination, and basophil activation tests.
Technical Innovations & Terminology
- Signal Processing: High-sensitivity PMTs (Photomultiplier Tubes) and digital signal processing with a sampling rate that prevents event loss at extreme flow rates.
- Spatial Separation: Three lasers focused at different points of the flow cell to minimize spectral overlap and simplify fluorescence compensation.
- Hydrodynamic Focusing: XN-series sheath flow technology ensures perfect cell centering, critical for low Coefficient of Variation (CV) and high resolution.
- Standardization & QC: Built-in tools for monitoring Mean Fluorescence Intensity (MFI) and channel sensitivity.
Technical Specifications
- Laser Configuration: 405 nm (100 mW), 488 nm (50 mW), 638 nm (40 mW).
- Fluorescence Sensitivity: <80 MESF (FITC), <30 MESF (PE).
- Particle Resolution: Detection from 0.2 µm (using SSC trigger).
- Throughput: Autoloader with continuous loading and active mixing to prevent cell sedimentation.
- Carry-over: < 0.1% due to automated probe washing cycles.